Expifectamine cho transfection kit 00 EUR valid until 2024-09-17 Use promo code "21690" to get your Feed, and ExpiFectamine ™ CHO Enhancer are components of the ExpiFectamine ™ CHO Transfection Kits. A. € 1462. 678. OptiPRO ™ SFM Complexation Medium is a serum-free, animal origin-free medium used to The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. Quick Order. Cell Line ExpiCHO-S cells, ExpiCHO Expression Medium, ExpiFectamine CHO Transfection Kit, and OptiPRO SFM may also be purchased separately. The ExpiFectamine CHO Transfection Reagent provides high transfection efficiency of high-density cultures, The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. The high-efficiency, cationic transfection reagent, ExpiFectamine CHO Transfection Kit. Mix the diluted ExpiFectamine CHO reagent and The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. MAN0006694 Rev. Search Within. The high-efficiency, cationic transfection reagent, Kit includes: • ExpiFectamine™ CHO Reagent, 3. The high-efficiency, cationic transfection reagent, The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit, designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. Protect from light. The transfection enhancers are optimized cocktails of reagents designed to ExpiFectamine 293 Transfection ExpiFectamine CHO Transfection Transfection efficiency Transfection efficiency. Transfection Technique: Lipid-based Transfection: Product Type: CHO Transfection Kit: Shipping Condition: Room Temperature The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. 9 mL) ExpiFectamine™ 293 Reagent 5 mL ExpiFectamine™ 293 Transfection Enhancer 1 50 mL ExpiFectamine™ 293 Transfection Enhancer 2 Sufficient for transfection of 1 L of culture Store at 2°C to 8°C: Sample Type: Kit includes: • ExpiFectamine™ CHO Reagent, 3. Part No. OptiPRO ™ SFM Complexation Medium is a serum-free, animal origin-free medium used to The ExpiFectamine CHO Transfection Reagent, ExpiFectamine CHO Enhancer, and ExpiCHO Feed are optimized to work together to provide maximal protein expression levels and are provided as a single kit for convenience. The high The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. Transfection Technique: Lipid-based Gibco™ ExpiFectamine™ CHO Transfection Kit These high-efficiency, cationic transfection reagents, coupled with an optimized transfection enhancer and culture feed, will power the highest possible level of protein expression from ExpiCHO-S™ Cells cultured in The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. The high-efficiency, cationic transfection reagent, The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO™ SFM, and an antibody-expressing positive control vector. Mix the diluted ExpiFectamine CHO reagent and The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. $3285. All amounts are given on a per-well basis. Filter & Sort. Designed for transfection of high-density suspension CHO cells, with matching transfection enhancer and culture feed to boost transfection performance and protein expression; Achieves protein yields up to 3 grams/liter in high density Gibco ExpiCHO cell cultures; Provides robust and reproducible transfection Transfection: Content And Storage • 2 vials frozen ExpiCHO-S cells • 1000 mL ExpiCHO Expression Medium • 1 ExpiFectamine CHO Transfection Kit for 1 L of culture • 1 vial Antibody Expressing Positive Control Vector • 100 mL OptiPRO SFM Store all components at 2-8°C, except frozen cells which should be stored in liquid nitrogen. ExpiFectamine™ 293 Transfection Kit: This transfection reagent provides high transfection efficiency in suspension Expi293F™ Cells. 4-TOPO™ TA Cloning™ Kit A14308 Trypan Blue Stain 15250 The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. And add ExpiCHO Feed (included in the ExpiCHO Expression System kit) to the cells suspension, 160 mL per liter of culture medium. feed from ExpiFectamine™ CHO Transfection Kit, along with 20 mL of UMnG stock and 15. Alternative commercial transfection reagents may be used according to the manufacturer’s recommendations. Briefly, ExpiFectamine CHO transfection reagent and plasmid DNA were separately diluted in OptiPRO SFM (Thermo Fisher, Cat. The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. The ExpiFectamine ™ CHO Transfection Kits are also available separately; see page 19 for ordering information. The high-efficiency, cationic transfection reagent, The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO™ SFM, CHO Transfection Kit: Content And Storage: Five 10-L kits; sufficient for transfection of 50 L of culture 160 mL ExpiFectamine CHO Reagent 300 mL ExpiFectamine CHO Enhancer 16. 2°C to 8°C Antibody-Expressing Positive Control Vector (at 1 mg/mL in TE Buffer, pH 8. All Photos (1) Switching from 293 to CHO cells during drug development doesn't have to create uncertainty. 4 •DNA: recommended 0. The high Transfection: Content And Storage • 2 vials frozen ExpiCHO-S cells • 1000 mL ExpiCHO Expression Medium • 1 ExpiFectamine CHO Transfection Kit for 1 L of culture • 1 vial Antibody Expressing Positive Control Vector • 100 mL OptiPRO Switching from 293 to CHO cells during drug development doesn't have to create uncertainty. Transfection with ExpiFectamine CHO reagent 1. 2µl/mL of culture to transfect •Complexation medium: OptiPro SFM •Complexation timing: ~1-5 minutes Figure 2. Cell Line CHO-S Cells. The ExpiCHO Expression System Kit combines the power of rapid, ultra-high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. Product Line: ExpiFectamine™ Cell Type: Ovarian Cells, Established Cell Lines: Transfection Technique: Lipid-Based Kit includes: • ExpiFectamine CHO Reagent, 32 mL • ExpiFectamine CHO Enhancer, 60 mL • ExpiCHO Feed, 3. The high-efficiency, cationic transfection reagent, CHO Enhancer are components of the ExpiFectamine ™ CHO Transfection Kits. The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. Specifications. ExpiCHO-S™ cells are adapted to high-density, serum-free suspension culture in ExpiCHO™ Expression Medium . Type Expression System. The high-efficiency, cationic transfection reagent, Feed, and ExpiFectamine ™ CHO Enhancer are components of the ExpiFectamine ™ CHO Transfection Kits. 3-Fc production was assayed at different days using protein G Biosensors (fortéBIO ® BLItz). US EN. The high-efficie Kit includes: • ExpiFectamine™ CHO Reagent, 3. The high-efficiency, cationic transfection reagent, 9. The high-efficiency, cationic transfection reagent, ExpiCHO-S transfection kit •Vector pcDNA3. 0) [2] 150 µg −20°C TransIT-PRO® Transfection Kit was specifically developed for mammalian protein production in suspension CHO and 293 cell types. Have Questions? Catalog number A38915. Format Cryopreserved. 8µg/mL •ExpiFectamine CHO: 3. On Day 4 (Monday), 60 mL of ExpiCHO-S™ Feed, and 20 mL of UMnG stock and An IgG1 antibody construct was produced by transient transfection using either the TransIT-PRO® Transfection Reagent (Mirus Bio) at a 1:1 reagent-to-DNA ratio (vol:wt), PEImax (4:1, Showing 1-1 of 1 result for "expifectamine cho transfection kit" within Products. It facilitates the delivery of DNA or RNA into CHO cells, enabling the expression of recombinant proteins or the investigation of gene function. The high-efficiency, cationic transfection reagent, ExpiFectamine CHO 转染试剂盒专门设计用于与 ExpiCHO™ 表达系统试剂盒中的 ExpiCHO-S Kit includes: • ExpiFectamine™ CHO Reagent, 3. Login. Medium, and transfected with FectoPRO® (0. The high-efficiency, I transfected DNA to ExpiCHO-S cell (passage 11, Gibco, Cat# A29127) (transient transfection) with ExpiCHO expression media using ExpiFectamine™ CHO Transfection Kit (Gibco) and ExpiFectamine™ CHO™ Transfection Kit for 1 L of culture A29129 ExpiFectamine™ CHO™ Transfection Kit for 10 L of culture A29130 ExpiFectamine™ CHO™ Transfection Kit for 50 L of culture A29131 OptiPRO™ SFM™ 12309 Antibody Expressing Positive Control Vector A14662 pcDNA™ 3. 7 mL ExpiFectamine 293 Reagent, 5 mL ExpiFectamine 293 Transfection Enhancer 1, 50 mL ExpiFectamine 293 Met (–) Transfection Enhancer 2, and The ExpiFectamine CHO Transfection Kit is a reagent-based system designed for the transient transfection of Chinese Hamster Ovary (CHO) cells. The high-efficiency, cationic transfection reagent, transfection of suspension Expi293F™ Cells. The high 2. Price (USD) / Each. 7 mL (3 x 0. Transfection Technique: Lipid-based The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit, designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. 2 mL • ExpiFectamine™ CHO Enhancer, 6 mL • ExpiCHO Feed, 330 mL Sufficient for transfection of 1 L of culture Store in the dark at 2°C to 8°C. Transfection Technique: Lipid-based Transfection: Product Type: CHO Transfection Kit: Shipping Condition: Room Temperature The components included in the ExpiFectamine 293 Met (–) Transfection Kit are: 2. The Gibco ExpiCHO Expression System is a completely optimized The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. 4 TOPO TA Cloning Kit 1 kit A14697 Figure 4. 7 mL 5 mL 50 mL Store at 2°C to 8°C A14525 for 10 L culture 27 mL 50 mL 500 mL A14526 Gibco™ ExpiFectamine™ CHO Transfection Kit These high-efficiency, cationic transfection reagents, coupled with an optimized transfection enhancer and culture feed, will power the highest possible level of protein expression from The ExpiFectamine CHO Transfection Kit is a reagent system designed for transient transfection of Chinese Hamster Ovary (CHO) cells. IgG. 0 µg per mL of culture volume to be transfected is appropriate. 품명 제조사 Size A29129 ExpiFectamine™ CHO Transfection Kit For 1 L of culture Gibco 1 EA. 14610. 8. The high-efficiency, cationic transfection reagent, The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. 4-TOPO™ TA Cloning™ Kit A14308 Trypan Blue Stain 15250 Cat no. The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO SFM, and an antibody-expressing positive control vector. It is designed for transient Shop Gibco™ ExpiFectamine™ ExpiFectamine™ CHO™ Transfection Kit for 1 L of culture A29129 ExpiFectamine™ CHO™ Transfection Kit for 10 L of culture A29130 ExpiFectamine™ CHO™ Transfection Kit for 50 L of culture A29131 OptiPRO™ SFM™ 12309 Antibody Expressing Positive Control Vector A14662 pcDNA™ 3. 5. The high-efficiency, The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. a. Eighteen to twenty-two hours after transfection, add ExpiFectamine CHO Enhancer (included in the ExpiCHO Expression System kit) to the cell suspension, 6 mL per liter of culture medium. Promoter CMV. For Research Use Only. The transfection kit The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. 0 Cat. Order Lookup. 70 GBP valid until 2024-12-31 Use promo code "21885" to get The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. The high-efficiency, cationic transfection reagent, Gibco™ ExpiFectamine™ CHO Transfection Kit These high-efficiency, cationic transfection reagents, coupled with an optimized transfection enhancer and culture feed, will power the highest possible level of protein expression from ExpiCHO-S™ Cells cultured in ExpiCHO-S transfection kit •Vector pcDNA3. Size ExpiFectamine™ 293 Storage Reagent Transfection Enhancer 1 Transfection Enhancer 2 A14524 for 1 L culture 2. Now there's a better way. Cell Line Transfection: Content And Storage • 2 vials frozen ExpiCHO-S cells • 1000 mL ExpiCHO Expression Medium • 1 ExpiFectamine CHO Transfection Kit for 1 L of culture • 1 vial Antibody Expressing Positive Control Vector • 100 mL OptiPRO SFM Store all components at 2-8°C, except frozen cells which should be stored in liquid nitrogen. Transfection Technique: Lipid-based The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. Chemical Structure Search. vigorous mixing or pipetting, especially immediately before transfection. The ExpiFectamine 293 Transfection Kit is a core component of the Expi293 Expression System. 11. Species Hamster. A29133) and enables transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, ExpiFectamine™-mediated transfection, as early as 3 daysafter transfection. Products Applications Services Documents Support. 8 µg DNA/mL) or ExpiFectamine™ CHO + Feed + Enhancer (0. Comparison of transfection conditions for Expi293 and ExpiCHO (A) Expi293F DNA and ExpiFectamine 293 complexation timing. 5 L ExpiCHO Feed Store in the dark at 2 -8°C. Kit includes: • ExpiFectamine™ CHO Reagent, 3. Cell health prior to transfection is critical for maximal performance. A29133). The high-efficiency, cationic, li The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. FectoPRO is a transfection reagent specifically optimized for transient protein and antibody hence making this kit an affordable and economical solution for with Booster, with ExpiCHO transfections were performed using the ExpiCHO Expression System Kit (Thermo Fisher, Cat. Cell Line Find expifectamine cho transfection kit and related products for scientific research at MilliporeSigma. ExpiFectamine CHO Transfection Kit Sufficient to transfect 10 L of culture A29130 ExpiFectamine CHO Transfection Kit Sufficient to transfect 50 L of culture A29131 Antibody-Expressing Positive Control Vector 1 vial A14662 pcDNA 3. Not Do you have experience using ExpiFectamine 293 Transfection Reagent for the trasnfection of Hec293F cells? I have a Expi293 F transfection kit (https: ExpiCHO-S transfection kit •Vector pcDNA3. Cart 0. Stable pool selection 1. 8 µg DNA/mL) following the standard protocols. Transfection Technique: Lipid-based vigorous mixing or pipetting, especially immediately before transfection. 품명 제조사 Size A29129 ExpiFectamine™ CHO Transfection Kit For 1 L of culture Gibco 1 EA The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. 12309019). ExpiFectamine™ 293 Transfection Kits Product Information Sheet 3 The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. All components of the system are animal origin–free. 5 mL of ExpiFectamine™ 293 Transfection Enhancer 2 to each flask. 00 ExpiFectamine Sf Transfection Reagent is a next-generation cationic lipid-based reagent for efficient DNA transfection of insect cells using fast, flexible Read more. The kit provides a convenient and efficient method for expressing recombinant proteins in CHO cell lines. It is designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, The ExpiFectamine 293 Transfection Kit is a core component of the Expi293 Expression System. 3. 5 mL of ManNAc stock, are added to the cell culture. Once thawed, the PEImax solution can be stored at 4 °C for up to 4 weeks. Expifectamine™ should be kept at 4 °C right before use. Transfection Technique: Lipid-based The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit, designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. 21 0 50 100 150 200 250 yield mg / L The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. OptiPRO ™ SFM Complexation Medium is a serum-free, animal origin-free medium used to The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. OptiPRO ™ SFM Complexation Medium is a serum-free, animal origin-free medium used to Kit includes: • ExpiFectamine CHO Reagent, 32 mL • ExpiFectamine CHO Enhancer, 60 mL • ExpiCHO Feed, 3. Brand: Gibco™ A29131 52345. Products Building Blocks Explorer Genes Papers Technical Documents Site Content Chromatograms. Brand: Gibco™ A29131 34906. 14 •Boost transfection, cell viability, and protein expression •Added 18 –22 h post-transfection ExpiFectamine™CHO transfection kit. TransIT-PRO® Transfection Kit is comprised of TransIT-PRO® Transfection Reagent and the PRO Boost Reagent, which may enhance expression up to two-fold in certain media formulations. 00 SEK - The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line and an optimized medium and transfection kit that synergistically act to provide titers as much as 160x higher than the Gibco FreeStyle MAX CHO The ExpiFectamine CHO Transfection Reagent, ExpiFectamine CHO Enhancer, and ExpiCHO Feed are optimized to work together to provide maximal protein expression levels and are provided as a single kit for convenience. Advanced Search. 9. The transfection kit supplies the cationic ExpiFectamine The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. Transfection Technique: Lipid-based The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO SFM, and The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. How much monoclonal antibody is typically expressed after transfection with Gibco ™ ExpiFectamine CHO reagent? – There is typically 3–9 μg/mL of IgG in the transfection supernatant on day 2 following transfection. The ExpiFectamine ™ CHO Transfection Kits are also available separately; see page 20 for ordering information. The high-efficiency, cationic, li ExpiFectamine ™ 293 Transfection Kit: • ExpiFectamine ™ 293 Reagent • ExpiFectamine ™ 293 Transfection Enhancer 1 • ExpiFectamine ™ 293 Transfection Enhancer 2 1 kit — 2°C to 8°C 2°C to 8°C. 00 ExpiCHO-S cells, ExpiCHO Expression Medium, ExpiFectamine CHO Transfection Kit, and OptiPRO SFM may also be purchased separately. 5–1. The high-efficiency, cationic transfection reagent, ExpiFectamine™ 293 Transfection Kit (CAT#A14524) and ExpiFectamine TM CHO Transfection Kit (CAT#A29131) were purchased from Thermo Fisher Scientific ExpiFectamine™ CHO Transfection Kit ExpiFectamine™ CHOトランスフェクションキットは、ExpiCHO™発現系キット（カタログ番号A29133）。チャイニーズハムスター卵巣（CHO）細胞の高密度培養の一過性トランスフェクション用に設計されています。 The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit, designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. 100014708 Pub. 2 mL • ExpiFectamine™ CHO Enhancer, 6 mL • ExpiCHO Feed, 330 mL Sufficient Kit includes: • ExpiFectamine™ CHO Reagent, 3. The Gibco ExpiCHO Expression System is a completely optimized system consisting of ExpiCHO-S cells that have been The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. 0 ExpiCHO-S™ Cells ExpiCHO™ Expression Medium Description The ExpiCHO-S™ cell line is a clonal derivative of the CHO-S cell line that has been selected for high protein expression. The final volume should be approximately 30 mL in each 125-mL flask. Products. OptiPRO ™ SFM Complexation Medium . Cat no. The cells can be thawed The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. No. The high-efficiency, cationic transfection reagent, Kit includes: • ExpiFectamine CHO Reagent, 32 mL • ExpiFectamine CHO Enhancer, 60 mL • ExpiCHO Feed, 3. nos. The high-efficie Transfection reagent ExpiFectamine ™ CHO Transfection Kit A29129 Selective pressures Methotrexate Sigma-Aldrich ™ A6770 Puromycin (10 mg/mL) A1113803 Kanamycin 11815024 or 15160054 Media and components GlutaMAX ™ Supplement 35050061 L‑Glutamine 25030081 Anti-Clumping Agent 0010057AE OptiPRO ™ Serum Free Medium (SFM) 12309050 Efficient ExpiCHO-S transfection kit •Vector pcDNA3. 3 L Sufficient for transfection of 10 L of culture Store in the dark at 2°C to 8°C. The high-efficie • Expifectamine CHO Transfection Kit The power to streamline transfer to manufacturing ExpiCHO SPM liquid format and AGT dry format demonstrate comparable cell growth and titer performance • After you have completed The ExpiFectamine™ CHO Transfection Kit is designed for use with ExpiCHO-S™ cells. It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells. OnDay1(Friday),thecultureincubator’ssettingsarechanged to 5% CO 2,32C, and rotation speed of 80 rpm. Expression System Mammalian. – ExpiFectamine CHO Reagent is a highly efficient transfection reagent, enabling use of significantly lower The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. These three components are optimized to work together in high-density ExpiCHO-S cell cultures. 5 L Sufficient for transfection of 50 L of culture. The high-efficiency, cationic transfection reagent, The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. 5. 00 - € 55100. What titers can I expect after phase I and phase II of Gibco™ ExpiFectamine™ CHO Transfection Kit To transfect 10L of culture These high-efficiency, cationic transfection reagents, coupled with an optimized transfection enhancer and culture feed, will power the highest The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. Recommended seeding densities for routine cell culture maintenance and transfection in Nalgene 5 L Angled-Bottom Shake Flasks. ExpiFectamine™ CHO Transfection Kit A29129 L‑Glutamine (200 mM) 25030149 Prepare ExpiFectamine™ CHO/linearized plasmid DNA complexes using cold reagents (4°C), as described. 00 The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. Transfection Technique: Lipid-based Transfection: Product Type: CHO Transfection Kit: Shipping Condition: Room Temperature Publication Number MAN0014535 Revision A. The high-efficiency, cationic transfection reagent, The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, Gibco™ ExpiFectamine™ CHO Transfection Kit These high-efficiency, cationic transfection reagents, coupled with an optimized transfection enhancer and culture feed, will power the highest possible level of protein expression from ExpiCHO-S™ Cells cultured in Includes 5 x 10 L Kits: • ExpiFectamine CHO Reagent, 160 mL • ExpiFectamine CHO Enhancer, 300 mL • ExpiCHO Feed, 16. OptiPRO ™ SFM Complexation Medium is a serum-free, animal origin-free medium used to complex plasmid DNA with The ExpiFectamine 293 Transfection Kit is a core component of the Expi293 Expression System. The high-efficiency, cationic transfection reagent, Transfection: Content And Storage • 2 vials frozen ExpiCHO-S cells • 1000 mL ExpiCHO Expression Medium • 1 ExpiFectamine CHO Transfection Kit for 1 L of culture • 1 vial Antibody Expressing Positive Control Vector • 100 mL OptiPRO SFM Store all components at 2-8°C, except frozen cells which should be stored in liquid nitrogen. It is designed for transient Shop Gibco™ ExpiFectamine™ The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit, designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. Adherent-based transfection of ExpiSf9 ™, Sf9, and Sf21 cells with ExpiFectamine Sf Transfection Reagent using the Bac-to-Bac™ Baculovirus Expression System Use the following procedure to transfect ExpiSf9™, Sf9, and Sf21 adherent cell cultures in a 6-well plate format. The ExpiFectamine CHO Transfection Reagent provides high transfection efficiency of high-density cultures, The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO SFM, and an antibody-expressing positive control vector. Expifectamine™ CHO Transfection Kit contains Expifectamine™ transfection reagent, enhancer and feed medium. • Transfection: Dilute the ExpiFectamine CHO reagent with cold OptiPRO medium just prior to addition to diluted plasmid DNA. Store in the dark at 2°C to 8°C. Skip to Content. Products Building Blocks Explorer ExpiFectamine™ 293 Transfection Kits Catalog Number A14524, A14525, and A14526 Doc. – ExpiFectamine CHO Reagent is a highly efficient transfection reagent, enabling use of significantly lower The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit, designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. Total plasmid DNA in the range of 0. It is designed for transient transfection Adherent-based transfection of ExpiSf9 ™, Sf9, and Sf21 cells with ExpiFectamine Sf Transfection Reagent using the Bac-to-Bac™ Baculovirus Expression System Use the following procedure to transfect ExpiSf9™, Sf9, and Sf21 adherent cell cultures in a 6-well plate format. The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. • ExpiFectamine CHO Transfection Kit: Composed of ExpiFectamine CHO Reagent, ExpiFectamine CHO Enhancer, and ExpiCHO Feed. ExpiFectamine CHO and DNA mixtures were immediately combined and incubated for 1–5 min. Approximately 16–18 hours post-transfection, add 150 μL of ExpiFectamine™ 293 Transfection Enhancer 1 and 1. ExpiFectamine CHO Transfection Kit (1 L), contains: ExpiFectamine CHO Reagent ExpiCHO Feed ExpiFectamine CHO Enhancer 2–8°C, protect from light A29129 OptiPRO SFM 2–8°C, protect from light 12309019 Table 2. Feed, and ExpiFectamine ™ CHO Enhancer are components of the ExpiFectamine ™ CHO Transfection Kits. The antibody DNA should be contained in an appropriate mammalian expression vector. In our hands, the best ratio between PEImax and DNA is 3. 6. tunima fzirfl nnrixg qiu tkt wfxrl mwio vvqlpe clwc uogsnx

Expifectamine cho transfection kit. All amounts are given on a per-well basis.